Нанополімери на основі полі-ДМАЕМ – перспективні вектори для доставки генетичного матеріалу у рослинні клітини

Abstract

Aims. Water soluble grafted polymers based on PDMAEMA (N,N-polydimethylaminoethyl metacrylate) are widely used in water systems for DNA and drug delivery to a target organs, tissues and cells. The significance of this research was in study of transformation efficiency and cytotoxic effects of novel synthesized PDMAEMA based nanopolymers, namely: BG-2, BG-2с, BG-2f, BG-2cq, BG-2cf, MP-1 and 83/6 on Nicotiana tabaccum L. protoplasts. Methods. In the process of this study there was developed a new method of protoplast transformation with the use of PDMAEMA based nanopolymers. Moreover, there were used standart plant cell culture and fluorescence light microsсopy methods. Results. The most effective for protoplast transformation was nanopolymers of 83/6 type in concentration of 0.1 %. Such transformation conditions showed the best efficiency and did not lead to significant effects on protoplast morphology. Conclusions. Finally, in a further studies related to the use of PDMAEMA based nanopolymers in plant transformation there should be used nanopolymers of 83/6 type in concentration of 0.1 %. Keywords: PDMAEMA, protoplasts, plasmid DNA, plant transformation.