Antibodies against benzo

Abstract

Aim: To evaluate the production of antibodies against benzo[a]pyrene (BF) (Ab1) and corresponding antiidiotypic antibodies (AM) in mice after immunization with BP-protein conjugate and in lung cancer patients. Materials and Methods: The Ab1 and AM levels were measured by non-competitive ELISA in blood serum of 10 mice immunized with BP-protein conjugate, and in blood serum of 288 healthy persons and 165 lung cancer patients. Results: The Ab] level of was 2-fold higher than Ab2 level in blood serum of BP-immunized mice. In lung cancer patients the Ab1 level was almost 3 times higher and the Ab2 level was by 30% higher than these indexes in healthy individuals. The Ab1/Ab2 ratio was 2 in BP-immunized mice and healthy individuals and l in lung cancer patients. Conclusion: Our data have shown that the Ab1/Ab2 ratio in lung cancer patients differ from that in healthy individuals and is close to the Ab1/Ab2 ratio in BP-immunized mouse. Key Words: benzo[a]pyrene, antibodies, lung cancer.