Дисбиотические аспекты патогенеза экспериментального неалкогольного стеатогепатита

Abstract

Высокожировой рацион в сочетании с кишечным дисбиозом вызывает развитие стеатогепатита, что указывает на целесообразность использования для его профилактики антидисбиотических средств.

Високожировий раціон в поєднанні з кишковим дисбіозом викликає розвиток стеатогепатиту, що вимагає застосовувати для його профілактики антидисбіотичні засоби.

Aim: To investigate of role of intestinal dysbiosis in nonalcoholic steatohepatitis. Materials and methods: Rats were feed high fat diet (fodder plus 15 % sunflower oil). One group of rats received high fat diet (HFD) on experimental intestinal dysbiosis, wich obtained by introduce of lincomycin in drinking water. The duration of experiment was 21 days. The content of triglyceride (TG), activity of elastase, urease, lysozyme and content of malondialdehide (MDA) were determined in liver homogenates. The content of MDA, activity of ALT and alcalin phosphatase (AP) were determined in serum. Degree of dysbiosis was determined by ratio of relative activities of urease and lysozyme. Results: HFD increased content of TG in liver, but no increased levels of hepatic marks (bilirubine, ALT and AP). Combination of HFD and intestinal dysbiosis increased level of TG in liver and in serum, increased levels of inflammation marks (MDA and elastase) and decreased of level of lysozyme in liver. Conclusion: Combination HFD and intestinal dysbiosis is essential to development of steatohepatitis. Antidysbiotic means is a need to prophylactic of nonalcoholic steatohepatitis.